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KMID : 1100820190090010017
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Laboratory Medicine Online
2019 Volume.9 No. 1 p.17 ~ p.21
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Evaluation of the Real-Q RV Detection Kit for the Identification of Viruses That Result in Respiratory Infections
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Lee Eun-Yup
Shin Sae-Am
Kim Mi-Young
Lee Young-Kyung
Kang Hee-Jung
Kim Hyun-Soo
Kim Jae-Seok
Song Won-Keun
Kim Han-Sung
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Abstract
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Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection (LoD) and usefulness of the Real-Q RV Detection kit (BioSewoom, Seoul, Korea). We measured the LoD of the Real-Q RV Detection kit using 10 strains of standard viruses. We then compared the detection results by the Allplex Respiratory Panel Assay kit (Seegene, Seoul, Korea) using 123 clinical specimens. The discrepant results were confirmed by sequencing. Among the 10 standard viruses, the LoD of human rhinovirus (HRV) was the lowest and that of parainfluenza virus 2 and 3 was relatively high as detected by Real-Q RV Detection kit. Agreements of the two kits ranged from 95.9% to 100%. Three specimens detected negative by the Allplex Respiratory Panel kit were detected as adenovirus (AdV) by the Real-Q RV Detection kit and were confirmed by sequencing. Similarly, a specimen detected negative by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. A specimen detected as human enterovirus by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. Real-Q RV Detection kit showed good diagnostic performance and can be useful for detecting major viruses that cause respiratory infections.
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KEYWORD
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Respiratory virus, Multiplex real-time PCR, Real-Q RV Detection kit
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