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KMID : 1100820200100030242
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Laboratory Medicine Online
2020 Volume.10 No. 3 p.242 ~ p.246
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Inhibition of Cross-Reactive Carbohydrate Determinant?Specific IgEs Reduce False Positive Reponses to Multiple Allergen Simultaneous Test?Immunoblot Assay: A Case Study
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Song Da-Young
Lee Joon-Hee
Kim Sung-Min
Jun Sun-Hee
Lee Kyung-Hoon
Song Sang-Hoon
Song Jung-Han
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Abstract
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Cross-reactive carbohydrate determinants (CCDs) are simple carbohydrates linked to amino acid chains; they are found in pollens, vegetable foods, insect, and Hymenoptera venoms and are broadly cross-reactive with CCD-specific IgE antibodies. A man in his fifties was evaluated using a multiple allergen simultaneous test?immunoblot assay. On the PROTIA Allergy-Q 64 inhalant panel (ProteomeTech, Korea), reactions to 37 of 59 antigens were observed except mammalian antigens, and cross-reactivity owing to anti-CCD antibodies was suspected. After ProGlycAn CCD-blocker (ProGlycAn, Austria) treatment, the patient exhibited no response to CCD allergens, and the number of allergens showing positive reactions was reduced to 15. We further tested a total of 7 samples from patients who were suspected to have CCD-related cross-reactivity. For these 8 patients, the average number of positive reactions to allergens was reduced from 33 (range 24-36) to 8 (range 0-19) after CCD-blocker treatment. We concluded that CCD-blocker treatment in sample with anti-CCD antibodies can reduce the false positive reponse and provide more specific information about allergens.
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KEYWORD
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Cross-reactive carbohydrates, Cross-reactive carbohydrate determinats, Allergens, Epitopes, False positives, IgE
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