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KMID : 1101420190510030360
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Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 3 p.360 ~ p.369
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Effect of Prostaglandin F2 Alpha on E-cadherin, N-cadherin and Cell Adhesion in Ovarian Luteal Theca Cells
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Lee Sang-Hee
Jung Bae-Dong
Lee Seung-Hyung
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Abstract
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Cadherins are essential transmembrane proteins that promote cell-cell adhesion and maintain the corpus luteum structure in the ovary. This study examined the influence of prostaglandin F2 alpha (PGF2?) on E-cadherin, N-cadherin, and adhesion in luteal theca cells (LTCs). The luteal cells were isolated from the mid-phase corpus luteum, and the LTCs were cultured separately from the luteal heterogeneous cells according to the morphology of the mesenchymal cells and to determine if steroidogenic and endothelial cells of LTCs, 3beta-hydroxysteroid dehydrogenase (3?-HSD), and vascular endothelial growth factor receptor 2 (VEGFR2) mRNA were used. The LTCs were then incubated in the culture medium supplemented with 0.01, 0.1, and 1.0 mM PGF2? for 24 h, and the E-cadherin and N-cadherin proteins in the LTCs were detected by confocal laser scanning microscopy. The results revealed 3?-HSD mRNA expression in the LTC but no VEGF2R mRNA expression. The E-cadherin and N-cadherin proteins of the LTCs were damaged in the 0.01, 0.1, and 1.0 mM PGF2? treatment groups, and the expression of the N-cadherin protein was reduced significantly in 0.01 mM PGF2? compared to the 0 mM PGF2? treatment groups (P＜0.05). In addition, the number of attached LTCs were significantly lower in the 0.01 mM PGF2? treatment group than in the 0 mM PGF2? treatment group (P＜0.05). In conclusion, PGF2? affected the disruption of cadherin proteins and cell adhesion in LTCs. These results may help better understand the cadherin and adhesion mechanism during corpus luteum regression in the ovary.
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KEYWORD
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Adhesion, Corpus luteum, E-cadherin, N-cadherin, Prostaglandin F2 alpha
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