KMID : 1120220120030040222
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Osong Public Health and Research Perspectives 2012 Volume.3 No. 4 p.222 ~ p.228
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Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8)
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Hong Sa-Hyun
Cha In-Jun Kim Nan-Ok Kim Seong-Han Jung Kyung-Tae Lee Je-Hee Kim Dong-Wook Park Mi-Sun Kang Yeon-Ho
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Abstract
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Objectives: We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay.
Methods: We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain.
Results: Approximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the ¡°not in COGs¡± category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains.
Conclusion: Our results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri.
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KEYWORD
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atypical, microarray, Shigella
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