KMID : 1123920020160061284
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Korean Journal of Oriental Physiology and Pathology 2002 Volume.16 No. 6 p.1284 ~ p.1290
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Scrophularia Buergeriana inhibits the Production of NO through the Suppression of NF-kB adivity in LPS-stimulated Mouse Peritoneal Macrophages
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Ha Mi-Suk
Kim Young-Hee Ko Woo-Shin Kim Han-Do
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Abstract
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Scrophularia buergeriana Miquel (Scrophulariaceae) has been used as an anti-inflammatory drug in the folk medicine recipe and been proved its anti-inflammatory effect in the oriental medicine. Since nitric oxide (NO) and superoxide anion (O/sub 2//sup -/) are ones of the major inflammatory parameters, we studied the effect of aqueous extracts of Scrophularia buergeriana (SB) on NO and O/sub 2//sup -/ production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. NO, O/sub 2//sup -/production and inducible NO synthase (iNOS) level were significantly reduced in LPS-activated macrophages by SB compared to those without. Electrophoretic mobility shift assay (EMSA) indicated that SB blocked the activation of NF-kB, which was considered to be a potential transcription factor for the iNOS expression. SB also blocked degradation of IkB¥á. Furthermore, IkB kinase alpha (IKK¥á), which phosphorylates serine residues of IkB directly, is inhibited by SB. These results suggest that SB could exert its anti-inflammatory actions by suppressing the activation of NF-kB through inhibition of IKK activity.
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KEYWORD
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Scrophularia buergeriana Mique, Superoxide, Nitric oxide (NO), Inducible nitric oxide synthase (iNOS), Nuclear Factor kappa B (NF-kB), Inhibitor kappa B(IkB), IkB kinase (IKK)
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