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KMID : 1130320080510121336
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Korean Journal of Pediatrics
2008 Volume.51 No. 12 p.1336 ~ p.1341
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Distribution of CD4＋CD25＋ T cells and graft-versus-host disease in human hematopoietic stem cell transplantation
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Lee Dae-Hyoung
Chung Nack-Gyun
Jeong Chang-Mo
Cho Bin
Kim Hack-Ki
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Abstract
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Purpose : This study aimed to determine the frequencies of CD4＋CD25＋ T cells in donor graft and peripheral blood CD4＋CD25＋ T cells in recipients after hematopoietic stem cell transplantation (HSCT) and their association with graft-versus-host disease (GVHD).
Methods : Seventeen children who underwent HSCT were investigated. CD4＋CD25＋ T cells in samples from donor grafts and recipient peripheral blood were assessed by flow cytometry at 1 and 3 months after transplantation.
Results : CD4＋CD25＋ T cell frequencies in the grafts showed no significant difference between patients with and without acute GVHD (0.90% vs. 1.06%, P=0.62). Absolute CD4＋CD25＋ T cell number in grafts were lower in patients with acute GVHD than in those without acute GVHD (6.18×105/kg vs. 25.85×105/kg, P=0.09). Patients without acute GVHD showed a significant decrease in peripheral blood CD4＋CD25＋ T cell percentage at 3 months compared to those at 1 month after HSCT (2.11% vs. 1.43%, P=0.028). However, in patients with acute GVHD, CD4＋CD25＋ T cell percentage at 3 months was not different from the corresponding percentage at 1 month after HSCT (2.47% vs. 2.30%, P=0.5).
Conclusion : The effect of frequencies of CD4＋CD25＋ T cells in donor grafts on acute GVHD after HSCT could not be identified, and the majority of peripheral blood CD4＋CD25＋ T cells in patients who underwent HSCT may be activated T cells related to acute GVHD rather than regulatory T cells. Further studies with additional markers for regulatory T cells are needed to validate our results.
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KEYWORD
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Regulatory T cell, Graft-versus-fost Disease, Allogeneic hematopoietic stem cell transplantation
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