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KMID : 1130320150580040142
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Korean Journal of Pediatrics
2015 Volume.58 No. 4 p.142 ~ p.147
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Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures
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Kim Seh-Hyun
Lee Woo-Soon
Lee Na-Mi
Chae Soo-Ahn
Yun Sin-Weon
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Abstract
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Purpose: The aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.
Methods: We prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.
Results: In the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P =0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P =0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P =0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P =0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P = 0.004).
Conclusion: Although only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.
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KEYWORD
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Hippocampus, Hypoxia, Brain
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