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KMID : 1130620160120040446
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Journal of Clinical Neurology
2016 Volume.12 No. 4 p.446 ~ p.451
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Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing
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Yao Ming
Zhou Jiali
Zhang Yinxin
Lv Xia
Sun Ruixue
Shen Ao
Ren Haitao
Cui Liying
Guan Hongzhi
Wu Honglong
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Abstract
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Background and Purpose: Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens.
Methods: This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis.
Results: Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases.
Conclusions: This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
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KEYWORD
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Listeria monocytogenes, meningoencephalitis, cerebrospinal fluid, next generation sequencing
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