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KMID : 1134820200490010019
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 1 p.19 ~ p.27
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Immunoregulatory Effects of Deer Antler Mixture Extract
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Choi Ji-Hae
Kim Na-Young
Kim Sun-Young
Park Hye-Ryung
Yu Kwang-Won
Lee Hyun-Soon
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Abstract
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A mixture of Cervus nippon (deer antler) and ten kinds of medicinal herbs was manufactured to develop new bioactive ingredients with immunoregulatory effects. Among them, both the ethanol extract and water extract of Acanthopanax sessiliflorum showed significant nitric oxide (NO) production and did not affect the Raw 264.7 cell viability. The optimal mixing ratio of C. nippon and A. sessiliflorum was found to be 1:1. The mixture (CME-PI, C. nippon mixture extract) showed higher immunoregulatory effects on the production of NO, IL-6, and TNF-α than a single treatment. In addition, the mRNA gene expression levels of IL-6, TNF-α, iNOS, and COX2 increased significantly, as determined by real-time PCR analysis. CME-PI showed considerably high proliferation activity in splenocytes, and cytokine production, such as IL-2 and IFN-γ, at a dose of 1,000 μg/mL. In an assay of the natural killer (NK) cell activity, the intravenous administration of CME-PI stimulated significant NK cytotoxicity against Yac-1 tumor cells in a dose-dependent manner. These results showed that CME-PI has superior potential as a material in high-added functional foods.
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KEYWORD
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deer antler, immunoregulation, Raw 264.7, Acanthopanax sessiliflorum, macrophage
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