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KMID : 1140120070120020136
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Cancer Prevention Research
2007 Volume.12 No. 2 p.136 ~ p.142
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Anticancer Effects of Sorghum Fractions in Vitro using SRB Assay
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Kweon Young-Mi
Kim Boh-Kyung
Lee Sook-Hee
Park Kun-Young
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Abstract
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Anticancer effects of various cereals were studied by surforhodamine B (SRB) assay on AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells. The methanol extracts of sorghum, millet and Job’s tears showed more than 50% of inhibition rate on the AGS cells and the HT-29 cells. Among the cereals, sorghum showed the highest inhibition rate on both the cancer cells. To separate and identify the anticancer compounds from sorghum, the methanol extract and five fractions (fr.) (hexane fr., dichloromethane fr., ethylacetate fr., butanol fr. and aqueous fr.) were prepared, and SRB assay on the AGS cells and the HT-29 cells were examined. The dichloromethane fr. and ethylacetate fr. of the sorghum exhibited strong cytotoxicity effects than other fractionated samples. The sorghum extract and
fractions inhibited survival of AGS cells and HT-29 cells in SRB assay. Among the fractions, dichloromethane fr. and ethylacetate fr. of the sorghum showed strong inhibitory effect on both AGS cells and HT-29 cells as concentration dependent-manner. Especially, dichloromethane fr. and ethylacetate fr. strongly inhibited the growth of AGS cells. This study resulted in sorghum, especially compounds in dichlromethane and ehtylacetate fractions exhihibited higest anticancer effect among the tested cereals. (Cancer Prev Res 12, 136-142, 2007)
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KEYWORD
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Sorghum, Cereals, SRB assay, AGS human gastric adenocarcinoma cells, HT-29 human colon carcinoma cells
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