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KMID : 1140120080130030169
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Cancer Prevention Research
2008 Volume.13 No. 3 p.169 ~ p.176
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Apoptosis by Quercetin through Induction of Bax and Activation of Caspase in Human Lung Carcinoma Cells
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Lee Jun-Hyuk
Hong Su-Hyun
Han Min-Ho
Jin Cheng-Yun
Choi Byung-Tae
Choi Yung-Hyun
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Abstract
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Quercetin, a plant flavonoid, is widely distributed in fruits and vegetables. This compound has various clinically relevant properties such as anti-oxidant, anti-inflammatory and anti-tumor activity. However, in spite of many reports, the molecular mechanism of quercetin-induced apoptosis in human lung carcinoma cells remains unclear. In this study, we examined the effect of quercetin on the growth and viability using an A549 human lung carcinoma cell line. It was found that quercetin could inhibit the cell growth in a concentration- and dose-dependent manner, which was associated with morphological changes and apoptotic cell death such as rounding up and formation of membrane bleb and spike, apoptotic bodies and increased populations of apoptotic sub-G1 phase. Apoptotic cell death of A549 cells by quercetin was connected with a up-regulation of pro-apoptotic Bax expression and tumor suppressor p53 without alteration of anti-apoptotic Bcl-xL expression. In addition, quercetin treatment induced the proteolytic activation of caspases (caspase-3, -8 and -9) and a concomitant degradation and/or inhibition of poly(ADP-ribose) polymerase (PARP) and β-catenin. Furthermore, caspase inhibitors significantly blocked quercetin-induced growth inhibition demonstrating the important role of caspases in the observed cytotoxic effect. (Cancer Prev Res 13, 169-176, 2008)
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KEYWORD
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Quercetin, A549, apoptosis, Bcl-2, caspase
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