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KMID : 1140120090140020132
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Cancer Prevention Research
2009 Volume.14 No. 2 p.132 ~ p.139
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Cytotoxic and Apoptotic Activity of Kochiae fructus Extracts on Human Colon Cancer Cell Lines
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Lee Jung-Min
Son Eun-Soon
Kim Ji-Hyun
Kim Hyun-Jung
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Abstract
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Natural products are widely used in pharmacological applications, due to their potential biological activities. Therefore, we established a screening of natural products as anticancer agents utilizing HT-29 human colon cancer cells. During the course of screening for anticancer agents, we have found a methanolic extracts of Kochiae fructus (KFM) from natural and it induced cytotoxicity and apoptosis in HT-29 cells. The KFM showed highly cytotoxic effects via the MTT reduction assay, LDH release assay, and colony formation assay. As expected, we also detected apoptotic bodies on Hoechst staining. To examine the functions on apoptosis, we used a flow cytometric analysis. The apoptotic cells were distributed according to the cell cycle phase shown by sub-G1 DNA content. These results indicate that KFM may contains bioactive materials and it could be a potential candidates as cancer chemotherapeutic against human colon cancer cells.
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KEYWORD
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Natural products, HT-29 cells, Kochiae fructus, Cytotoxicity, Apoptosis
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