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KMID : 1140120090140030233
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Cancer Prevention Research
2009 Volume.14 No. 3 p.233 ~ p.241
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Inhibition of Cyclooxygenase-2 Activity and Prostaglandin E2 Production through Down-regulation of NF-κB Activity by Peptide Derived from Soy Bean
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Park Cheol
Choi Byung-Tae
Choi Yung-Hyun
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Abstract
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Frequent consumption of soy and soy-based products is associated with reduced inflammation and cancer incidence. Pro-inflammatory mediators, such as prostaglandin E2 (PGE2) and cyclooxygenases-2 (COX-2), play pivotal roles in normal as well as transformed cells. In this study, we examined the effect of peptide [Leu-Leu-Pro-His-His (LLPHH)] derived from soy bean on phorbol 12-myristate 13-acetate (PMA)-induced inflammatory responses using human lymphatic U937 cell model. Treatment of PMA significantly induced COX-2 expression and PGE2 production in U937 cells. However, the pretreatment with LLPHH markedly inhibited the PMA-induced COX-2 expression as well as PGE2 production in a dose-dependent manner. Moreover, the LLPHH prevented the nuclear translocation of nuclear factor kappaB (NF-κB p65) stimulated by PMA treatment. However, the elevated levels of early growth response gene-1 (Egr-1) expression by PAM were not reduced by the pretreatment of LLPHH. Taken together, the present data indicate that the LLPHH derived from soy bean exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-kB signaling pathway. These findings support the hypothesis that the soy peptides reduce the risk of tumorigenesis possibly by suppressing inflammatory responses.
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KEYWORD
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Soy peptide, LLPHH, Cyclooxygenases-2, Prostaglandin E2, NF-kB
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