KMID : 1140220190240020123
|
|
´ëÇѾϿ¹¹æÇÐȸÁö 2019 Volume.24 No. 2 p.123 ~ p.128
|
|
Esculetin Prevents the Induction of Matrix Metalloproteinase-1 by Hydrogen Peroxide in Skin Keratinocytes
|
|
Zhen Ao Xuan
Piao Mei Jing Kang Kyoung-Ah Fernando Pincha Devage Sameera Madushan Kang Hee-Kyoung Koh Young-Sang Hyun Jin-Won
|
|
Abstract
|
|
|
Background: Reactive oxygen species (ROS) are involved in various cellular diseases. Excessive ROS can cause intracellular oxidative stress, resulting in a calcium imbalance and even aging. In this study, we evaluated the protective effect of esculetin on oxidative stress-induced aging in human HaCaT keratinocytes.
Methods: Human keratinocytes were pretreated with esculetin for 30 minutes and treated with H2O2. Then, the protective effects on oxidative stress-induced matrix metalloproteinase (MMP)-1 were detected by Flou-4-AM staining, reverse transcription-PCR, Western blotting, and quantitative fluorescence assay.
Results: Esculetin prevented H2O2-induced aging by inhibiting MMP-1 mRNA, protein, and activity levels. In addition, esculetin decreased abnormal levels of phospho-MEK1, phospho-ERK1/2, phospho-SEK1, phospho-JNK1/2, c-Fos, and phospho-c-Jun and inhibited activator protein 1 binding activity.
Conclusions: Esculetin prevented excessive levels of intracellular calcium and reduced the expression levels of aging-related proteins.
|
|
KEYWORD
|
|
Esculetin, Reactive oxygen species, Matrix metalloproteinase-1, Aging
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|