KMID : 1143720100060020089
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Korean Journal of Neurotrauma 2010 Volume.6 No. 2 p.89 ~ p.96
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In Vitro Compressive Neural Injuries Produced by Centrifugal Acceleration
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Kim Jong-Hyun
Moon Hong-Joo Kim Joo-Han Kwon Taek-Hyun Park Youn-Kwan Chung Hung-Seob
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Abstract
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Objective:We describe a technique to induce in vitro traumatic brain injury in an organotypic slice culture (OTC).
Methods:Centrifugal forces were applied to rat hippocampal slices at strengths ranging from 300 to 7,500 g.
Results:The degree of neural injury correlated with the forces applied. A morphometric analysis of hippocampal CA area revealed significant tissue expansion. A graded posttraumatic decrease in evoked field potentials was recorded from acutely prepared slices. Immediate posttraumatic cell death was identified at the base of the slice where it was attached to the porous membrane. Delayed cell death and secondary damage induced by serum deprivation, excitotoxicity, hypoxic insults, and additional impacts were investigated and observed by conventional fluorescent microscopy.
Conclusion:Although this static compressive model of neural injury differs in impact duration from the clinical situation, it may be useful for neurotrauma research because it is accessible, easily adjustable, and reliable.
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KEYWORD
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Centrifugal force, In vitro model, Organotypic slice, Static compressoin, Traumatic brain injury
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