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KMID : 1145120160140030301
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 3 p.301 ~ p.308
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Anti-oxidant and Anti-melanogenic Activity of the Methanol Extract of Pine Cone
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Lee Ah-Reum
Roh Seong-Soo
Lee Eun-Sook
Min Yu-Hong
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Abstract
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Purpose: This study was conducted to evaluate anti-oxidant and anti-melanogenic activities of pine cone extract from Pinus densiflora.
Methods: Pine cone extract used in this experiment was extracted with methanol at room temperature for 24 h. Anti-oxidant activities were measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and nitro blue tetrazolium chloride (NBT) assay. Moreover, the inhibitory effects of pine cone methanol extract on tyrosinase and melanin synthesis in murine B16-F10 melanoma cells were studied. In addition, cell viability was investigated by MTT assay.
Results: The results showed that the pine cone methanol extract strongly reduced the DPPH free radical activity (IC50=9.57±1.24 μg/mL). However, its activity was lower than the activity of L-ascorbic acid (positive control, IC50=1.28±0.03 μg/mL). In NBT assay, the pine cone methanol extract exhibited more superoxide anion radical scavenging activity (IC50=3.33±0.28 μg/mL), compared with L-ascorbic acid (IC50=73.25±1.02 μg/mL). When treated with 3,4-Dihydroxy-L-phenylalanine (L-DOPA) as the substrate of tyrosinase, the extract inhibited the tyrosinase activity with an IC50 value of 106.15±1.69 μg/mL, lower effect than that of kojic acid (IC50=3.12±0.68 μg/mL). In murine B16-F10 cells, the pine cone methanol extract significantly suppressed melanogenesis in a concentration-dependent manner without cytotoxicity.
Conclusion: The results indicated that pine cone extract could be potentially valuable as a new potent depigmentation agent.
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KEYWORD
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Pine cone, Methanol extract, Anti-oxidant activity, Anti-melanogenic activity, Cytotoxicity
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