KMID : 1145720150030010007
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Korean Herbal Medicine Informatics 2015 Volume.3 No. 1 p.7 ~ p.14
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Introducing for Healthy Seed Selection and Germination Test of Medicine Herbs using by Tetrazolium (2,3,5-triphenyl tetrazolium chloride) Test
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Lee Ka-Youn
Min Ji-Yoon Moon Byeong-Cheol Kang Young-Min
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Abstract
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Propagation technology and evaluation of seed viability is a highly demanded technique in the production of Korean herbal medicine. To produce Raphanus seeds as the examples of Korean herbal medicine, seed germination improvement is very important. In this study, tetrazolium test was used because of rapid and simple method. After the selection of seed groups by the tetrazolium test, healthy seeds were A group and bad seeds were B group. The germination of seeds in A group were significantly different (alpha=0.05) from the germination of seeds in B group the given time. In addition, B seed group could not be cultured and propagated a long time because of tetrazolium chemical damage and had bad embryo physiologically. Also, nondestructive evaluation of viability for individual seeds as X-ray seed analysis or hyperspectral reflectance imaging system was required in future study. These seed analytical studies by tetrazolium test will be improved technology for reproductive medicinal herbs including Raphanus seeds.
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KEYWORD
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Propagation Technology, Raphanus Seed, Seed Germination Improvement, Tetrazolium Test
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