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KMID : 1147220190200020044
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Journal of Biomedical and Translational Research
2019 Volume.20 No. 2 p.44 ~ p.52
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Novel porcine model of acute myocardial infarction using polyethylene terephthalate
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Kim Yong-Sook
Kim Nan-Yeol
Bae In-Ho
Park Jun-Kyu
Park Dae-Sung
Shim Jae-Won
Kang Wan-Suk
Kim Han-Byul
Song Bong-Seok
Koo Bon-Sang
Jeong Kang-Jin
Kim Sun-Uk
Kim Hyun-Kuk
Kim Sung-Soo
Kim Min-Chul
Sim Doo-Sun
Hong Young-Joon
Ahn Young-Keun
Lim Kyung-Seob
Jeong Myung-Ho
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Abstract
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Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5-triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.
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KEYWORD
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polyethylene terephthalate, porcine model, myocardial infarction, interventional cardiology, cardiovascular disease
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