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KMID : 1148920120460010020
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Nuclear Medicine and Molecular Imaging
2012 Volume.46 No. 1 p.20 ~ p.26
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Development of a 68Ga-Fluorinated Porphyrin Complex as a Possible PET Imaging Agent
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Fazaeli Yousef
Jalilian Amir R.
Amini Mostafa M.
Ardaneh Khosro
Rahiminejad Ali
Bolourinovin Fatemeh
Moradkhani Sedigheh
Majdabadi Abbas
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Abstract
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Aim: Due to the interesting pharmacologic properties of porphyrins, the idea of developing a possible tumor imaging agent using PET by incorporating 68Ga into a suitable porphyrin ligand was investigated.
Methods: 68Ga-labeled 5,10,15,20-tetrakis(pentafluoro-13 phenyl) porphyrin (68Ga-TFPP) was prepared using freshly eluted [68Ga]GaCl3 obtained from a 68Ge/68Ga generator developed in-house and 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin (H2TFPP) for 60 min at 100°C.
Results: The complex was prepared with high radiochemical purity (>99% ITLC, >99% HPLC, specific activity: 13?14 GBq/mmol). Stability of the complex was checked in the final formulation and in human serum for 5 h. The partition coefficient was calculated for the compound (log P = 0.62). The biodistribution of the labeled compound in vital organs of Swiss mice bearing fibrosarcoma tumors was studied using scarification studies and SPECT imaging up to 1 h. The complex was mostly washed out from the circulation through kidneys and liver. The tumor-to-muscle ratio 1 h post injection was 5.13.
Conclusion: The radiolabeled porphyrin complex demonstrated potential for further imaging studies in other tumor models.
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KEYWORD
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Porphyrins, Ga-68, Fibrosarcoma, Biodistribution, Co-incidence
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