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KMID : 1148920150490030208
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Nuclear Medicine and Molecular Imaging
2015 Volume.49 No. 3 p.208 ~ p.216
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Preparation and Evaluation of 68Ga-ECC as a PET Renal Imaging Agent
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Mirzaei Alireza
Jalilian Amir Reza
Aghanejad Ayuob
Mazidi Mohammad
Yousefnia Hassan
Shabani Gholamali
Ardaneh Khosro
Geramifar Parham
Beiki Davood
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Abstract
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Purpose: Development of a gallium-68-labeled renal tracer can be a good substitute for Tc-99m, a known SPECT tracer. In this study, effort was made to develop 68Ga-ethylenecysteamine cysteine (68Ga-ECC).
Methods: Ga-ECC was prepared using generator-based 68GaCl3 and ethylenecysteamine cysteine (ECC) at optimized conditions. Stability of the complex was checked in human serum followed by partition coefficient determination of the tracer. The biodistribution of the tracer in rats was studied using tissue counting and PET/CT imaging up to 120 min.
Results: Ga-ECC was prepared at optimized conditions in 15 min at 90 °C (radiochemical purity ?97?±?0.88 % ITLC, >99 % HPLC, specific activity: 210?±?5 GBq/mM). 68Ga-ECC was a water-soluble complex based on partition coefficient data (log P; ?1.378) and was stable in the presence of human serum for 2 h at 37 °C. The biodistribution of the tracer demonstrated high kidney excretion of the tracer in 10?20 min. The SUVmax ratios of the liver to left kidney were 0.38 and 0.39 for 30 and 90 min, respectively, indicating high kidney uptake.
Conclusion: Initial biodistribution results showed significant kidney and urinary excretion of the tracer comparable to that of the homologous 99mTc compound. The complex could be a possible PET kidney imaging agent with a fast imaging time.
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KEYWORD
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68Ga, Ethylenecysteamine cysteine, Biodistribution, Renal agent, PET/CT
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