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KMID : 1149220210290040277
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Korean Journal of Oriental Medical Prescription
2021 Volume.29 No. 4 p.277 ~ p.283
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Effect of Bojungikgi-tang on cytochrome P450 and LKB1-AMPK anti-oxidant signaling pathway
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Song Yu-Rim
Park Sun-Dong
Kim Young-Woo
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Abstract
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Objectives : We investigated the effects of Bojungikgi-tang (BJIGT) on P450 cytochrome enzyme and oxidative stress in the cells.
Methods : We enrolled the HepG2 hepatocyte cell line to assess MTT assay, flow cytometer, and immunoblotting analysis. Expression of CYP450 was confirmed by immunoblotting analysis in the Huh7 cell line.
Results : We determined that BJIKT markdely changed the expression of the CYP2C19, CYP2D6, and CYP2E1. Moreover, BJIKT inhibited the cell toxicity induced by arachidonic acid + iron treatment, as assessed by FACS analysis. BJIKT induced AMPK activation, which increased the phophorylation of ACC.
Conclusions : This study verified the effects of BJIKT, on P450, ROS production, mitochondrial damage and AMPK signaling pathway, which might give us the scientific information about the traditional herbal prescription.
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KEYWORD
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Bojungikgi-tang, AMPK, LKB1, CYP450
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