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KMID : 1149220220300010011
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Korean Journal of Oriental Medical Prescription
2022 Volume.30 No. 1 p.11 ~ p.18
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Crab Water Extrct Ameliorates Reflux Esophagitis in Rats
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Nam Hyeon-Hwa
Seo Yun-Soo
Song Jun-Ho
Lee A-Yeong
Noh Pu-Reum
Moon Byeong-Cheol
Lee Ji-Hye
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Abstract
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Objectives: In this study, we aim to demonstrate an effect of crab water extract (CWE) on reflux esophagitis (RE) using lipopolysaccharide (LPS)-induced Raw 264.7 cell and rat model.
Methods: To investigate on LPS-induced Raw 264.7 cell, CWE was co-treated with LPS. CWE suppressed Nitric Oxide (NO) production which increased by LPS treatment. Also, CWE showed no cytotoxicity at the concentrations range from 500 ㎍/㎖ to 2000 ㎍/㎖. Next, to investigate the protective effects of CWE on RE rat model, eighteen rats were divided in to three groups: sham group, reflux esophagitis group, and reflux esophagitis pre-treated with 100 mg/kg CWE 1 h before surgery. RE was induced by a pylorus and forestomach ligation operation and all rats were sacrificed after 4 h 30 min from surgery.
Results: In gross examination, the CWE administration attenuated esophageal mucosal injury upon histological evaluation of reflux esophagus of rats. The CWE downregulated the expression levels of proteins related to inflammation, such as COX-2 and TNF-α in the esophagus tissue. In addition, the CWE suppressed the NF-κB and IκB-α activation.
Conclusions: Based on these findings, we concluded that CWE could possess protective effect against damage to the esophagus due to reflux esophagitis.
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KEYWORD
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anti-inflammation, crab, NF-κB, reflux esophagitis
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