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KMID : 1149220220300040259
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Korean Journal of Oriental Medical Prescription
2022 Volume.30 No. 4 p.259 ~ p.267
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Erythrocyte Micronucleus Test of Pomace Schisandra chinensis Extracts Using ICR Mouse
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Kim Seok-Ho
Kim Sun-Youn
Kim Young-Suk
Lim Jong-Min
Ku Bon-Hwa
Kwak Kyeung-Tae
Jeon Byeong-Yeob
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Abstract
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Objectives : In this study, erythrocyte micronucleus test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis.
Methods : The micronucleus test was performed according to the 'OECD Guidelines'. Including the negative control group(0 mg/kg) and the positive control group(CPA 70 mg/kg), pomace Schisandra chinensis extracts were orally administered to ICR mouse at doses of 500, 1,000, and 2,000 mg/kg. After sacrificing the experimental animals bone marrow cells were collected and micronucleated polychromatic erythrocyte were counted. And genetic toxicity was confirmed according to the frequency of micronucleus.
Results : As a result of the micronucleus test, there were no changes in body weight, clinical signs, or death in any group. But, a significant increase was observed in the frequency of micronucleated polychromatic erythrocyte among polychromatic erythrocytes in the positive control group administered with CPA compared to the negative control group(p<0.05). Whereas, no significant increase was observed in the group administered with pomace Schisandra chinensis extracts compared to the negative control group.
Conclusions : Pomace Schisandra chinensis extracts did not induce micronucleus in bone marrow cells of ICR mouse up to a concentration of 2,000 mg/kg, and it was judged that no genetic toxicity was observed.
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KEYWORD
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Pomace Schisandra chinensis, up-cycling, genetic toxicity, erythrocyte micronucleus test, OECD
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