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KMID : 1156220080340020170
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Journal of Environmental Health Sciences
2008 Volume.34 No. 2 p.170 ~ p.174
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Molecular Epidemiological Analysis of Outbreaks to Pathogenic Salmonella othmarschen Using PFGE
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Bang Sun-Jae
Park Kwang-Hee
Park Myung-Ki
Kwon Yion-Ok
Ko Hoan-Uck
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Abstract
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The strains of Salmonella othmarschen and Norovirus isolated from outbreak A, the funeral ceremonies incidents in Guri-shi of Gyeonggido in, 2007, were subjected to genotyping by using PFGE and extraction of RNA by using RTPCR. 24 Salmonella othmarschen were isolated from 37 cases (patients 12 cases, cookers 4 cases, foods 7cases, knife, kitchen board, dish towel 4 cases, etc) and PFGE patterns showed that 22 strains isolated from the same incident were almost of the same pattern and 2 strains exhibited 50% of the pattern. Antimicrobial susceptibility patterns were such that there were 22 case susceptibility patterns and 2 case resistance patterns (strain 4: AM,CF, CIP, NA, TE TIC and strain 13: CF,CZ). Norovirus G1 (1case), Norovirus G2 (7cases), Norovirus G1,G2 (1case) were detected by using RTPCR. The fine typing ability and reliability of PFGE and BioNumerics are helpful for establishing out the foodborne pathogens of outbreaks.
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KEYWORD
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Salmonella othmarchen, Norovirus, PFGE, antimicrobial susceptibility RT-PCR
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