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KMID : 1156220110370060429
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Journal of Environmental Health Sciences
2011 Volume.37 No. 6 p.429 ~ p.439
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Analysis of Micronuclei and Its Association with Genetic Polymorphisms in Hospital Workers Exposed to Ethylene Oxide
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Lee Sun-Yeong
Kim Yang-Jee
Choi Young-Joo
Lee Joong-Won
Lee Young-Hyun
Shin Mi-Yeon
Kim Won
Yoon Chung-Sik
Kim Sung-Kyoon
Chung Hai-Won
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Abstract
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Objectives: Ethylene oxide (EtO) is classified as a human carcinogen, but EtO is still widely used to sterilize heat-sensitive materials in hospitals. Employees working around sterilizers are exposed to EtO after sterilization. The aim of the present study was to assess the exposure of EtO level, coupled with occupationally induced micronuclei from hospital workers. The influence of genetic polymorphisms of detoxifying genes (GSTT1 and GSTM1) and DNA repair genes (XRCC1 and XRCC3) on the frequencies of micronuclei in relation to exposure of EtO was also investigated.
Methods: The study population was composed of 35 occupationally exposed workers to EtO, 18 student controls and 44 unexposed hospital controls in Korea. Exposure to EtO is measured by passive personal samplers. We analyzed the frequencies of micronuclei by performing cytokinesis-block micronucleus assay (CBMN assay) and GSTM1, GSTT1, XRCC1, and XRCC3 were also genotyped by performing polymerase chain reaction?restriction fragment length polymorphism (PCR?RFLP).
Results: The frequencies of micronuclei in EtO exposure group, student controls and hospital controls were 18.00 ± 7.73, 10.47 ± 7.96 and 13.86 ± 6.35 respectively and their differences were statistically significant, but no significant differences according to the level of EtO were observed. There was a dose-response relationship between the frequencies of micronuclei and cumulative dose of EtO, but no significantly differences were observed. We also investigated the influence of genetic polymorphisms (GSTM1, GSTT1, XRCC1, and XRCC3) on the frequencies of micronuclei, but there were no differences in the frequencies of micronuclei by genetic polymorphisms.
Conclusions: The frequencies of micronuclei in EtO exposure group was significantly higher than control
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KEYWORD
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Ethylene oxide (EtO), Genetic polymorphism, Glutathione S-transferase (GST), Hospital workers, Micronuclei (MN), X-ray repair cross-complementing group (XRCC)
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