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KMID : 1156220130390020166
Journal of Environmental Health Sciences
2013 Volume.39 No. 2 p.166 ~ p.177
Molecular Epidemiology of Legionella pneumophila Isolated from Water Supply Systems in Seoul, Korea
Jeon Su-Jin

Jung Ji-Hun
Seung Hyun-Jung
Kim Chang-Kyu
Jin Young-Hee
Oh Young-Hee
Choi Sung-Min
Chae Young-Zoo
Abstract
Objectives: The genus Legionella is common in aquatic environments. Some species of Legionella are recognized as potential opportunistic pathogens for human, notably Legionella pneumophila that causes, Legionellosis. Thus, we investigated the contamination of Legionella pneumophila on water supply systems in Seoul, including cooling towers, public baths, hospitals and fountains.
Methods: The existence of 16S rRNA and mip gene of L. pneumophila was confirmed in the genome of the
isolated strains by PCR.

Results: During the summer season of 2010 and 2011, Legionella pneumophila were detected from 163 samples (21.1%) out of 772 samples collected. Among the 163 strains of L. pneumophila, eighty one isolates belonged to serogroup 1 (57.4%), 23 isolates were serogroup 5 (16.3%), 21 isolates were serogroup 6 (14.9%), 8 isolates were serogroup 2 (5.79%), and 8 isolates were identified in serogroup 3 (5.7%). Through PFGE (pulsed-field gel electrophoresis) analysis using Sfi I, genetic types of L. pneumophila were classified into five (A to E) patterns by the band similarity with excess of 70% from public baths.

Conclusions: The PFGE patterns of the serotypes showed a tendency for diversity of L. pneumophila. Our results suggest the existence of serological and genetic diversity among the L. pneumophila isolates.
KEYWORD
Legionella pneumophila, mip, Pulsed-field gel electrophoresis (PFGE)
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