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KMID : 1156220160420040274
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Journal of Environmental Health Sciences
2016 Volume.42 No. 4 p.274 ~ p.279
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Analysis of Bacterial Diversity in Water from the Han River Water Source Protection Area via a Pyrosequencing Assay
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Kim Hee-Jung
Kwon Dug-In
Kim Chang-Soo
Lee Si-Won
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Abstract
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Objectives: We investigated bacterial diversity in the Han River water resource protection area in order to provide basic microbiological information on the drinking water safety of the Seoul metropolitan region.
Methods: Samples were collected in the spring and winter, but not during the rainy season. Pyrosequencing, gene amplification, and extraction of nucleic acids were employed in this study.
Results: In total, 57 and 48 operational taxonomic units were respectively analyzed in samples collected during spring and winter. Proteobacteria were predominant in all samples. The samples contained phylogenetically diverse bacterial communities, with eleven major phyla and 36 genera. Cyanobacteria were predominant in the spring samples, but not in the winter samples. The predominant species in the samples collected during both seasons belonged to the genus Aquamicrobium and Bradyrhizobium. Moreover, no pathogenic bacteria were detected in the samples.
Conclusion: Proteobacteria were predominant in the samples from the Han River water source protection area. Cyanobacteria were more predominant in the spring samples than in the winter samples, but Aquamicrobium and Bradyrhizobium were predominant in both sampling seasons.
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KEYWORD
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Drinking water safety, Han River, Pyrosequencing, water resource protection area
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