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KMID : 1156220170430010077
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Journal of Environmental Health Sciences
2017 Volume.43 No. 1 p.77 ~ p.86
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A Permeation Characteristics Study of Water- or Oil-soluble Substances through Condition Setting for the In Vitro Skin Absorption Method
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Seo Ji-Eun
Lee Jin-Ho
Kim Bae-Hwan
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Abstract
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Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oil-soluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428.
Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS.
Results: The permeation rate increased dose-dependently, and the permeation orders were KeraSkinTM > hairless
mouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials.
Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as 'fast' and 'moderate' compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.
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KEYWORD
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Franz cell , skin permeation , alternative method , condition setting
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