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KMID : 1157520130090030163
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International Journal of Clinical Preventive Dentistry
2013 Volume.9 No. 3 p.163 ~ p.168
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Identification Quantity of Actinomyces in Children Saliva with Black Stain in Tooth Enamel Surface
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Ike Siti Indiarti
Yuke Rustan
Sarworini Bagio Budiardjo
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Abstract
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Objective: The aim of this study is to differentiate the quantity of Actinomyces on saliva of children with and without black stain at the surface of tooth enamel.
Methods: The subject is chosen from children aged 4-11 years with black stain more that 8 surfaces of tooth enamel and without black stain. The saliva is taken by instructing subject to expectorate into a sterile container and inserted into a sterile plastic with Oxoid Anaerobic Gas pack to keep the anaerobic condition when transported to laboratory. In the laboratory, serial dilution was done and sample was inserted into a plate which contains Difco Actinomyces Isolate agar. Put the plate into an anaerobic jar and incubated in incubator. From the plate, subculture identification was done to identify the morphol-ogy of Actinomyces. The colony of Actinomyces on the plate was count with colony counter using the colony forming unit method.
Results: The result was analyzed with t-test two groups unpaired and concluded that the quantity of Actinomyces on child-ren's saliva with and without black stain of the enamel surface is not different.
Conclusion: The results of this study indicate the quantity of Actinomyces bacteria in saliva of children with black stain is higher than in children without black stain saliva. But the results of the statistical test using t-test, it was found that the quan-tity of actinomyces in saliva of children with black stain and without black stain did not differ significantly.
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KEYWORD
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actinomyces, black stain, saliva
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