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KMID : 1160220200480030219
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Mycobiology
2020 Volume.48 No. 3 p.219 ~ p.227
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The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells
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Chiang Cheng-Yen
Hsu Kai-Di
Lin Yen-Yi
Hsieh Chang-Wei
Liu Jui-Ming
Lu Tze-Ying
Cheng Kuan-Chen
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Abstract
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Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer.
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KEYWORD
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Prostate cancer, Ganoderma formosanum, cell cycle arrest, cyclin, apoptosis
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