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KMID : 1161320200350040289
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Journal of Animal Reproduciton and Biotechnology
2020 Volume.35 No. 4 p.289 ~ p.296
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Expression profile of spermatogenesis associated genes in male germ cells during postnatal development in mice
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Ahn Jin-Seop
Ryu Hyun-Sung
Jung Sang-Eun
Shin Beom-Jin
Won Jong-Hyun
Um Tea-Gun
Oh Hui-Jo
Kim Seo-Hee
Ryu Buom-Yong
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Abstract
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Spermatogonial stem cells are self-renewal and differentiate into sperm in post-pubertal mammals. There exists a balance between the self-renewal and differentiation in the testes. Spermatogonial stem cells make up only 0.03% of testicular cells in adult mice. These cells maintain sperm production by differentiating after puberty. Therefore, analyzing the expression of genes associated with spermatogenesis is critical for understanding differentiation. The present study aimed to establish the postnatal period of cells in relation to spermatogenesis. To study the expression of differentiated and undifferentiated marker genes in enriched spermatogonial stem cells, in vitro culture was performed and cells from pup (6?8-day-old) and adult (4-months-old) testicular tissues were isolated. As a result, undifferentiated genes, Pax7, Plzf, GFRa1, Etv5 and Bcl6b, were highly increased in cultured spermaotogonial stem cells compared with pup and adult testicular cells. On the other hands, differentiated gene, c-kit was highly increased in adult testicular cells, Also Stra8 gene was highly increased in pup and adult testicular cells. This study provides a better understanding of spermatogenesis-associated gene expression during postnatal periods.
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KEYWORD
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adult testicular cells, gene expression, pup testicular cells, spermatogenesis, spermatogonial stem cells
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