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KMID : 1161320230380010026
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Journal of Animal Reproduciton and Biotechnology
2023 Volume.38 No. 1 p.26 ~ p.31
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In vitro culture of chicken embryonic stem cell-like cells
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Lee Bo-Ram
Yang Hyeon
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Abstract
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Chicken embryonic stem (ES) cells have great potential and provide a powerful tool to investigate embryonic development and to manipulate genetic modification in a genome. However, very limited studies are available on the functional characterization and robust expansion of chicken ES cells compared to other species. Here, we have developed a method to generate chicken embryonic stem cell-like cells under pluripotent culture conditions. The chicken embryonic stem cell-like cells were cultivated long-term over several passages of culture without loss of pluripotency in vitro and had the specific expression of key stem cell markers. Furthermore, they showed severe changes in morphology and a significant reduction in pluripotent genes after siRNA-mediated NANOG knockdown. Collectively, these results demonstrate the efficient generation of chicken embryonic stem cell-like cells from EGK stage X blastoderm-derived singularized cells and will facilitate their potential use for various purposes, such as biobanking genetic materials and understanding stemness in the fields of animal biotechnology.
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KEYWORD
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characterization, chicken, embryonic stem cell-like cells, knockdown
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