|
KMID : 1161420130160110968
|
|
Journal of Medicinal Food
2013 Volume.16 No. 11 p.968 ~ p.976
|
|
|
Blueberry (Vaccinium virgatum) Leaf Extracts Protect Against Aβ-Induced Cytotoxicity and Cognitive Impairment
|
|
Jeong Hee-Rok
Jo Yu-Na
Jeong Ji-Hee
Kim Hyeon-Ju
Kim Man-Jo
Heo Ho-Jin
|
|
|
Abstract
|
|
|
|
The ethylacetate (EtOAc) fraction of blueberry leaf extract was investigated to examine the in vivo antiamnesic effects against amyloid β protein (Aβ)-induced learning and memory deficit. The fraction showed the highest antioxidant activities, and the generation of intracellular reactive oxygen species was significantly decreased. Cell viability assays revealed the in vitro cytoprotective effects of the fraction, and the cytoplasmic lactate dehydrogenase release into the medium was dose-dependently inhibited. In addition, a chlorogenic acid was identified as a predominant phenolic compound by high-performance liquid chromatography analysis. Antiamnesic effects were evaluated by using in vivo the Y-maze and passive avoidance tests, and preadministration of the fraction attenuated Aβ-induced memory impairment in both in vivo experiments. Acetylcholinesterase prepared from mice brain was inhibited by the fraction, and malondialdehyde generation in the brain homogenate was also decreased. These findings suggest that the EtOAc fraction of blueberry leaf extract could possess a wide range of physiological effects against neurodegenerative diseases.
|
|
|
KEYWORD
|
|
|
antiamnesic activity, blueberry leaf, chlorogenic acid, Vaccinium virgatum
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|