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KMID : 1161420140170060670
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Journal of Medicinal Food
2014 Volume.17 No. 6 p.670 ~ p.677
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Antioxidant and Hepatoprotective Effects of Naringenin and Its β-Cyclodextrin Formulation in Mice Intoxicated with Carbon Tetrachloride: A Comparative Study
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Hermenean Anca
Ardelean Aurel
Stan Miruna
Hadaruga Nicoleta
Mihali Ciprian-Valentin
Costache Marieta
Dinischiotu Anca
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Abstract
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The present study evaluated the antioxidant and hepatoprotective effects of the flavonoid naringenin (NGN) and its β-cyclodextrin formulation at a dose of 50?mg/kg b.w. The assessment was done by the investigation of serum-enzymatic and liver antioxidant activity, histopathological and ultrastructural changes in male Swiss mice, which were subjected to acute experimental intoxication with CCl4. Formulated and free flavonoid were orally given to mice for 7 days and then were intraperitoneally injected with 1.0?mL/kg CCl4 on the 8th day. After 24?h of CCl4 administration, an increase in the levels of transaminases aspartate aminotransferase and alanine aminotransferase activities and malondialdehyde concentration occurred and a significant decrease in superoxide dismutase, catalase glutathione-peroxidase activities, and glutathione levels was detected as well. These were accompanied by extended centrilobular necrosis, steatosis, fibrosis, and an altered ultrastructure of hepatocytes. Pretreatment with formulated or free flavonoid retained the biochemical markers to control values. Histopathological and electron-microscopic examination confirmed the biochemical results. In conclusion, both NGN and NGN/β-cyclodextrin complex showed antioxidant and hepatoprotective effects against injuries induced by CCl4.
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KEYWORD
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antioxidant activity, CCl4, hepatoprotection, naringenin
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