KMID : 1161420160190121196
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Journal of Medicinal Food 2016 Volume.19 No. 12 p.1196 ~ p.1203
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FlexPro MD, a Mixture of Krill Oil, Astaxanthin, and Hyaluronic Acid, Suppresses Lipopolysaccharide-Induced Inflammatory Cytokine Production Through Inhibition of NF-¥êB
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Park Doo-Ri
Ko Ryeo-Jin Kwon Suk-Hyung Min Bok-Kee Yun Seong-Ho Kim Manh-Heun Minatelli John Hill Stephen Lee Soo-Young
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Abstract
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FlexPro MD¢ç (FP-MD), a novel multi-ingredient dietary supplement formulation, has been demonstrated to relieve knee joint pain in humans. However, the mechanisms of action responsible for the activity of FP-MD have not been elucidated. In this study, we show the anti-inflammatory effects of FP-MD in RAW264.7 macrophage cells and mice challenged with lipopolysaccharide (LPS). FP-MD significantly inhibited the mRNA levels of pro-inflammatory cytokines, including interleukin-6 (IL-6), tumor necrosis factor-¥á (TNF-¥á), and IL-1¥â. In contrast, it elevated the mRNA levels of anti-inflammatory cytokine IL-10 in LPS-stimulated RAW264.7 cells. FP-MD markedly reduced LPS-induced phosphorylation levels of nuclear factor-¥êB (NF-¥êB) p65 and inhibitor of ¥êB-¥á (I¥êB-¥á). Importantly, the anti-inflammatory effects of FP-MD were demonstrated in mice with LPS-induced inflammatory arthritis in which FP-MD significantly reduced the expression levels of pro-inflammatory cytokines and inflammatory markers. Thus, this study suggests that FP-MD has anti-inflammatory effects by inhibiting NF-¥êB that may offer a molecular basis for its pain relief property.
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KEYWORD
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astaxanthin, cytokines, NF-¥êB
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