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KMID : 1161519980020010087
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Animal Cells and Systems
1998 Volume.2 No. 1 p.87 ~ p.91
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Requirement of protein kinase C pathway during progesterone-induced oocyte maturation in amphibian, Rana dybowskii
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Bandyopadhyay Jaya
Bandyopadhyay Arun
Kang Hae-Mook
Kwon Hyuk-Bang
Choi Hueng-Sik
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Abstract
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The present study investigated the involvement of the phospholipase C (PLC) and protein kinase C (PKC) signaling pathways during progesterone?induced meiotic maturation in amphibian (Rana dybowskii) oocytes. Progesterone?induced germinal vesicle breakdown (GVBD) of oocytes was significantly inhibited by a PKC inhibitor, staurosporine and a PLC inhibitor, U73122, in a dose?dependent manner. In contrast, U73343, an inactive analogue of U73122, was ineffective in suppressing GVBD. PKC activity in oocytes reached a maximum level at 30 min after progesterone stimulation and this elevated PKC activity was effectively suppressed by U73122 or staurosporine, suggesting that the activation of PKC enzyme is closely linked to PLC signaling during oocyte maturation. In addition, these inhibitors blocked the maturation promoting factor (MPF) activity which appeared in oocytes in response to progesterone, suggesting that PKC activation is an important signal for MPF activity. Therefore, this study demonstrates that the activation of PKC via PLC signaling is directly linked to an intracellular protein kinase cascade related to the appearance of MPF activity during meiotic maturation in amphibian (Rana dybowskii) oocytes.
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KEYWORD
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Oocyte maturation, Protein kinase C, Staurosporine, U73122, Maturation-promoting factor, Rana dybowskii, Amphibian
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