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KMID : 1161519980020010113
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Animal Cells and Systems
1998 Volume.2 No. 1 p.113 ~ p.116
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Isolation and characterization of the ribosomal protein 46 gene in Drosophila melanogaster
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Kim Joon-Ki
Lee Chung-Choo
Chung Ki-Wha
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Abstract
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A cDNA clone coding for ribosomal protein 46 (rp46) which is a component of 60S ribosomal large subunit has been identified from Drosophila melanogaster. A cDNA clone encoding S. cerevisiae rp46 was used as a probe to screen a Drosophila larvae cDNA library. The DNA sequence analysis revealed that the cDNA coding for Drosophila rp46 contains a complete reading frame of 153 nucleotides coding for 51 amino acids. The deduced amino acid sequence showed 71?75% homology with those of other eukaryotic organisms. Northern blot analysis showed that about 1?kb rp46 transcripts are abundant throughout fly development. Whole mount embryonic mRNA in situ hybridization also showed no preferential distribution of the transcripts to any specific region. The chromosomal in situ hybridization revealed that the identified gene is localized at position 60C on the right arm of the second polytene chromosome with a possibility of single copy
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KEYWORD
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Ribosome, Ribosomal protein 46, Drosophila melanogaster
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