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KMID : 1161519990030010047
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Animal Cells and Systems
1999 Volume.3 No. 1 p.47 ~ p.52
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Characteristics of antifreeze protein-1 induced during low temperature acclimation in the protaetia brevitarsis (coleoptera; Cetonidae) larva
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Lee Hyung-Chul
Yoo Chong-Myung
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Abstract
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Change of proteins was confirmed during low temperature acclimation of overwintering larva, and some biochemical characteristics of the induced antifreeze protein?1 (AFP?1) were investigated in Protaetia brevitarsis. As the freezing point depression by the action of induced AFPs, a considerable thermal hysteresis was observed in the haemolymph and in partially purified proteins. AFP?1 was purified from the cold acclimation larvae by ammonium sulfate precipitation, ion exchange chromatography, gel permeation chromatography, and electroelution. The purified AFP?1 was determined to be a glycoprotein (approximately 320 kDa, pl 5.8) composed of a single type of subunit (80 kDa). The high contents of hydrophilic amino acids (Asp, Glu, Lys, Asn, Gln, Arg, Ser, Thr) were also confirmed, showing similarity with antifreeze proteins from other insects
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KEYWORD
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Antifreeze protein, Thermal hysteresis, Protaetia brevitarsis
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