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KMID : 1161519990030010069
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Animal Cells and Systems
1999 Volume.3 No. 1 p.69 ~ p.72
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Identification of a tandemly repeated DNA sequence using combined RAPD and FISH in welsh onion (allium Fistulosum)
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Seo Bong-Bo
Do Geum-Sook
Lee Seon-Hee
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Abstract
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A tandemly repeated DNA sequence was identified and characterized by the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5?GAAACGGGTG?3’, in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C?banded constitutive heterochromatin in the terminal regions of both arms of all 16 chromosomes
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KEYWORD
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Tandemly repeated DNA sequence, RAPD, FISH, Nucleotide sequence
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