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KMID : 1161520170210020108
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Animal Cells and Systems
2017 Volume.21 No. 2 p.108 ~ p.114
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Functional restoration of replicative senescent mesenchymal stem cells by the brown alga Undaria pinnatifida
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Jeong Sin-Gu
Oh Youn-Seo
Joe I-Seul
Jeong So-Young
Cho Hyo-Moon
Lee Jun-Sik
Oh Won-Keun
Cho Tae-Oh
Cho Goang-Won
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Abstract
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The brown alga Undaria pinnatifida, which is called Mi-Yoek in Korea, has been traditionally consumed as a health food in East Asian countries. Recent studies have reported that U. pinnatifida has beneficial effects on arteriosclerosis, inflammation, fat metabolism, and tumors. In this study, we examined the anti-senescence effects of ethanol extracts of U. pinnatifida (UP-Ex) in human bone marrow mesenchymal stem cells (hBM-MSCs). UP-Ex protected hBM-MSCs against oxidative injury, as determined by MTT assays. This effect was confirmed by immunoblot analysis of the oxidation-sensitive protein p53 and the apoptotic protein cleaved caspase-3. Excessive intracellular reactive oxygen species (ROS) accumulation induced by oxidative stress was moderated in UP-Ex-treated hBM-MSCs (UP-Ex-MSCs). Similarly, expression of the ROS-scavenging enzymes superoxide dismutase 1 (SOD1), SOD2, and catalase was recovered in UP-Ex-MSCs. Excessive ROS induced by long-term cell expansion (passage 17) was significantly decreased along with restoration of the senescence proteins p53, p21, and p16 in UP-Ex-MSCs. UP-Ex treatment also improved the ability of these replicative, senescent hBM-MSCs (passage 17) to differentiate into osteocytes or adipocytes, suggesting that UP-Ex ameliorates the functional decline of senescent stem cells and may provide better therapeutic efficacy in stem cell therapy.
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KEYWORD
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Undaria pinnatifida, replicative senescence, long-term expansion, anti-oxidation, mesenchymal stromal cells
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