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KMID : 1161820210050020023
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Journal of Natural Healing
2021 Volume.5 No. 2 p.23 ~ p.31
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The Composition of Free Amino Acids and Phenolic Acids in Lac Pigment Extracts, and Physiological Activity of Cosmetics
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Jang Ah-Ram
Song Yeon-Sook
Lee Keun-Kwang
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Abstract
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The composition and content of some free amino acids and non-protein amino acid derivatives of the hot water and methanol extract of the Lac pigment, and the composition and content of phenolic compounds were analyzed, and the physiological activity of some skin beauty cosmetics were as follows. Free amino acids were not detected in hot water and methanol extracts, respectively, 2 kinds of protein non-constituent amino acid derivatives were detected in hot water extract and 3 kinds were detected in methanol extract. In the composition and content analysis of phenolic acid and flavonoids, 2 types of phenolic acid were detected in hot water extract and 5 types were detected in methanol extract. In addition, flavonoids were not detected in hot water extract, but rutin and taxifolin were slightly present in methanol extract. DPPH radical scavenging activity was slightly higher in methanol extract than in hot water extract at 40.43 ± 0.21%, and in tyrosinase inhibitory activity, methanol extract was approximately twice as high as in hot water extract. In
addition, cytotoxicity to the HaCaT cell line was not observed at all treatment concentrations, and cell growth tended to increase as the treatment concentration increased. In the treatment concentration of these extracts of 100㎍/ml, the cell activity of about 30-60% was higher than that of the control. Therefore, according to the above results, the hot water and methanol extract of Lac pigment contains some non-protein derivatives, phenolic acid and flavonoid components, and it appears to have some physiological activity for skin beauty. It is believed that it is valuable for use as an ingredient, but it is considered that additional research is needed continuously in order to actually use it.
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KEYWORD
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Lac pigment, free amino acid, phenolic acid and flavonoid, DPPH, tyrosinase
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