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KMID : 1188320140080020140
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Gut and Liver
2014 Volume.8 No. 2 p.140 ~ p.147
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Effects of DA-9701, a Novel Prokinetic Agent, on Phosphorylated Extracellular Signal-Regulated Kinase Expression in the Dorsal Root Ganglion and Spinal Cord Induced by Colorectal Distension in Rats
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Lee Sang-Pyo
Lee Kang-Nyeong
Lee Oh-Young
Lee Hang-Lak
Jun Dae-Won
Yoon Byung-Chul
Choi Ho-Soon
Hwang Se-Jin
Lee Seo-Eun
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Abstract
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Background/Aims:DA-9701, a standardized extract of Pharbitis Semen and Corydalis Tuber, is a new prokinetic agent that exhibits an analgesic effect on the abdomen. We investigated whether DA-9701 affects visceral pain induced by colorectal distension (CRD) in rats.
Methods:A total of 21 rats were divided into three groups: group A (no CRD+no drug), group B (CRD+no drug), and group C (CRD+DA-9701). Expression of pain-related factors, substance P (SP), c-fos, and phosphorylated extracellular signal-regulated kinase (p-ERK) in the dorsal root ganglion (DRG) and spinal cord was determined by immunohistochemical staining and Western blotting.
Results:The proportions of neurons in the DRG and spinal cord expressing SP, c-fos, and p-ERK were higher in group B than in group A. In the group C, the proportion of neurons in the DRG and spinal cord expressing p-ERK was lower than that in group B. Western blot results for p-ERK in the spinal cord indicated a higher level of expression in group B than in group A and a lower level of expression in group C than in group B.
Conclusions:DA-9701 may decrease visceral pain via the downregulation of p-ERK in the DRG and spinal cord.
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KEYWORD
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DA-9701, Gastrointestinal diseases, Visceral hypersensitivity, Colorectal distension, Phosphorylated extracellular signal-regulated kinase
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