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KMID : 1189420070200010080
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2007 Volume.20 No. 1 p.80 ~ p.98
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Study on the Anti-oxydative, Anti-tumor effect of Whakijogyungtang
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Yu Mi-Kyung
Kim Jong-Han
Park Su-Yeon
Choi Jung-Hwa
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Abstract
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Objectives: This study was carried out to evaluate anti-oxydative, anti-tumor effect for clinical application of Whakijogyungtang (WJT)
Results: 1. DPPH radical scavenging activities of WJT water extracts(Exts) were in proportion as concentration of WJT.(3 %) 2. ABTS+ scavenging activities of WJT water Exts were more effective in high density.(3 %, 10 : %, 30 100 : ) 3. Hydrogen peroxide scavenging activities of WJT water Exts were effective.(3 %, 10 : %, 30 % 100 : %) 4. Anti oxidative effects against linoleic acid were not effective. 5. The generation of O₂in S-180 cells were according to the concentration of WJT water Exts, specially effective over 100 ug/ml concentration. 6. The SOD activities in S-180 cells were in proportion as cytotoxicity against S-180 cells of WJT water Exts. 7. The GPx activities in S-180 cells were in proportion as cytotoxicity against S-180 cells of WJT water Exts(more effective on 300 ug/ml and 1000 ug/ml concentration), but the catalase activities in S-180 cells were not effective. 8. The results of activites against multi-drug-resistance(MDR) of WJT were as follows. 1) In water Exts from WJT, cytotoxicity against AML-2/D100 with vincristine was more effective than without vincristine( 183.58 ), Cross resistance(CR:3.85) was not effective, and anti-MDR activites(RF) was effective.(RF:3.85) 2) In hexane fraction, cytotoxicity against AML-2/D100 with vincristine was more effective than without vincristine and anti-MDR activites(RF) was effective.(RF:4.61) 3) In chloroform fraction, the cytotoxicity against AML-2/WT and AML-2/D100 was not effective. 4) In ethyl acetate fraction, cytotoxicity against AML-2/D100 with vincristine was more effective than without vincristine , Cross resistance(CR:0.53) was not effective, and anti-MDR activites(RF) was effective.(RF:2) 5) In butanol fraction, the cytotoxicity against AML-2/WT and AML-2/D100 was not effective. 6) In H₂O fraction, the cytotoxicity against AML-2/WT and AML-2/D100 was not effective.
Conclusion: These result suggest that WJT has antioxidative effects, anti-tumor effects by apoptosis of free radical activity, and anti-MDR activites(especially hexane and ethyl acetate fraction).
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KEYWORD
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Anti-oxydative, Anti-tumor, Whakijogyungtang
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