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KMID : 1189420130260020045
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2013 Volume.26 No. 2 p.45 ~ p.57
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Roots of Daucus carota sativa abrogates acute phase of Inflammation by the Inhibition of NO and Pro-Inflammatory Cytokine Production
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Lee Dong-Jin
Jee Seon-Young
Park Sang-Mi
Hwangbo Min
Jung Tae-Young
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Abstract
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Objectives : Daucus carota sativa has been frequently used as food supplements in many of the Asian countries, and a nutritional medical drug in traditional medicine. This research investigated the effects of Daucus carota sativa extract (DCE) on acute phases of inflammation in Raw 264.7 cells treated with lipopolysaccharide (LPS) in terms of the inhibition of nitric oxide (NO), prostaglandin E2 (PGE2) and pro-inflammatory cytokines production.
Methods : NO, PGE2, tumor necrosis factor (TNF)-α, interleukin-1β and interleukin-6 contents were assayed by ELISA, and expressions of inflammation-related proteins such as inducible NO synthase (iNOS) were determined by immunoblot analyses.
Results : DCE treatment attenuated the LPS ability to increase the productions of NO and PGE2 as well as the protein level of iNOS in a concentration-dependent manner. Consistently, treatment of the cells with DCE suppressed the production of TNF-α, interleukin-1β and interleukin-6. DCE also caused decreases of inhibitor of κBα phosphorylation induced by LPS in the cells, which means DCE inhibition of NF-κB activity. Furthermore, DCE blocked LPS-induced phosphorylation of p38 and SAPK/JNK.
Conclusion : This study showing here may be of help to understand the action mechanism of DCE, and provide the information for the medical use of Daucus carota sativa for the inflammatory disease.
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KEYWORD
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Daucus carota sativa, iNOS, TNF-α, Interleukin-β, Interleukin-6, MAPK
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