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KMID : 1189420190320040001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2019 Volume.32 No. 4 p.1 ~ p.12
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Anticancer and Signaling Mechanisms of Biologically Active Substances from Orostachys japonicus through Arrest of Cell cycle in Human Melanoma Cells
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Ryu Deok-Hyun
Ryu Deok-Seon
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Abstract
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Objectives : The purpose of this study was to identify the anticancer effect of biological substances of ethylacetate(EtOAc) fraction from Orostachys japonicus(OJEF), their effect on human melanoma A375 cells and the related molecular mechanisms.
Methods : The MTS assay was used to confirm the inhibition of cancer cell proliferation in A375 cells. And the MUSE™ analyzer was used to determine the ability of OJEF to induce cell cycle arrest. Western blotting was used to determine the changes in protein expression in A375 cells after treatment with OJEF.
Results : OJEF showed cytotoxicity to A375 cells. And cell cycle arrest occurred in G1 phase and G2/M phase owing to inhibition of CDK1, cyclin B1, CDK4, and cyclin D, which are related to cell cycle regulation and cell division control.
Conclusion : OJEF is effective in regulating cell cycle of human melanoma cells and thus can be a good theraputic agent to treat patients with melanoma.
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KEYWORD
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Melanoma, Orostachys Japonicus, Anticancer, Cell Cycle Arrest
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