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KMID : 1189420210340040012
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2021 Volume.34 No. 4 p.12 ~ p.23
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Anti-aging and Anti-oxidative Effect of Naesohwangryun-tang(NSHRT)
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Oh Sol-Ra
Nam Sang-Woo
Song Young-Chae
Kim Hee-Tack
Kim Yong-Min
Lee Chun-Il
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Abstract
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Objectives : This study is to investigate the anti-aging and antioxidant effects of Naesohwangryeontang(NSHRT) extract in Hs68 fibroblasts.
Methods : The fibroblast cell line Hs68 cells were used and MTT analysis was performed to measure the cell viability of Naesohwangryeon-tang, and then MMP-1 was induced with the cytokine TNF-α to confirm the anti-aging effect through real-time PCR and ELISA. In addition, ROS was induced with the TNF-α to confirm the anti-oxidant effect through DCF-DA. The signaling pathway according to the antioxidant effect was performed by western blot.
Results : Naesohwangryeon-tang did not show cytotoxicity at all concentrations(100-800㎍/㎖) treated on Hs68 cells, and the inhibitory effect of MMP-1 was also confirmed. In addition, it was confirmed that the intracellular ROS increased by TNF-α was decreased by Naesohwangryeon-tang, and it was confirmed that the expression of NQO1 was increased in a concentration-dependent manner of Naesohwangryeon-tang.
Conclusions : These results suggest that Naesohwangryeon-tang can be used as an anti-aging material by demonstrating the anti-aging and antioxidant effects of Naesohwangryeon-tang.
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KEYWORD
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Anti-oxidative, Anti-aging, Naesohwangryun-tang, MMP-1
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