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KMID : 1189420210340040024
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2021 Volume.34 No. 4 p.24 ~ p.36
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Anti-oxidative Effect of Chungpyesagan-tang in LPS Induced RAW 264.7 Cells
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Jeon Bo-Hee
Kim Tae-Jun
Kim Hee-Taek
Kim Yong-Min
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Abstract
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Objectives : This study was conducted to confirm the anti-oxidative effect of Chungpyesagan-tang(CPSGT) extract.
Methods : In this study, MTT assay was performed to confirm cell viability, and DPPH and ABTS were performed to confirm radical scavenging ability. The ROS scavenging ability and the protective effect against DNA damage were confirmed by 2,7-dichlorofluorescin diacetate(DCF-DA) and 4′,6-diamidino-2-phenylindole(DAPI) staining and comet assay. mRNA expression of Heme oxygenase-1(HO-1) was measured by real-time PCR, and expression of HO-1 and Kelch-like ECH-associated protein 1(Keap1) proteins was measured by western blot.
Results : CPSGT was not cytotoxic at 50-400㎍/㎖. The radical scavenging activity was increased, and the ROS scavenging activity and the protective effect against DNA damage were increased compared to the LPS-treated group. The mRNA expression and protein expression of HO-1 were increased in a concentration-dependent manner. The protein expression level of Keap1 was decreased in a concentration-dependent manner.
Conclusion : This suggests that CPSGT has an antioxidant effect and can be used as a potential material for skin diseases.
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KEYWORD
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Chungpyesagan-tang(CPSGT), Anti-oxidative, ROS, Heme-oxygenase 1, DNA damage
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