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KMID : 1195620190120010086
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Clinical and Experimental Otorhinolaryngology
2019 Volume.12 No. 1 p.86 ~ p.94
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Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
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Batioglu-Karaaltin Aysegul
Ovali Ercument
Karaaltin Mehmet V
Yener Murat
Yilmaz Mehmet
Eyupoglu Fatma
Yilmaz Yetkin Zeki
Bozkurt Erol Rustu
Demir Necdet
Konuk Esma
Bozdag Ergun Sureyya
Yigit Ozgur
Cansiz Harun
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Abstract
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Objectives: The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques.
Methods: Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up.
Results: DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L).
Conclusion: Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.
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KEYWORD
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Trachea, Scaffold, Freeze Drying, Tissue Engineering, Deoxycholic Acid
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