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KMID : 1200820200200040581
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Oriental Pharmacy and Experimental Medicine
2020 Volume.20 No. 4 p.581 ~ p.589
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Antimicrobial activity of nanoemulsion encapsulated with polyphenon 60 and ciprofloxacin for the treatment of urinary tract infection
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Kaur Atinderpal
Gabrani Reema
Dang Shweta
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Abstract
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Ciprofloxacin (Cipro) is a broad spectrum synthetic antibiotic that can be used to treat many bacterial infections. But the rising antibiotic resistance has limited its use. Combining it with a natural antimicrobial compound could enhance the therapeutic value as well as effectiveness against resistant bacteria. In the present work, a nanoemulsion loaded with polyphenon 60 and Cipro was analyzed by using different assays such as microtiter dish biofilm assay to check the antibacterial potential, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT assay) to analyze cyto-toxicity of formulation, adhesion assay and confocal laser microscopy to identify the mechanism of antibacterial effect of the formulation. Further, growth of Escherichia coli in the presence of nanoemulsion was also recorded. The antibacterial study showed that the nanoemulsion inhibited the formation of biofilm with average % inhibition of 89% as compared to aqueous formulation showing average % inhibition of 67% at their minimum inhibitory concentrations. The cell toxicity studies confirmed that the nano formulation was not toxic against vero cells with % viability of ~?78% than the aqueous solution ~?61% and placebo ~?37%. The nanoemulsion was also able to inhibit the bacterial adhesion to mammalian cells and growth of E. coli at the 2nd hour of inoculation. Confocal laser scanning microscopy showed that the formulation exert its antibacterial activity by disrupting the bacterial cell membrane. Hence, the results concluded that the developed nanoemulsion loaded with polyphenon 60 and Cipro had enhanced antibacterial activity and it could further be explored for the management of urinary tract infection.
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KEYWORD
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Adhesion assay, Biofilm, Confocal laser microscopy, Uropathogens
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