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KMID : 1204320200360010039
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Laboratory Animal Research
2020 Volume.36 No. 1 p.39 ~ p.39
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A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources
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Kang Kyung-Ku
Kim Young-In
Seo Min-Soo
Sung Soo-Eun
Choi Joo-Hee
Lee Si-Joon
Jung Young-Suk
Cho Joon-Young
Hwang Dae-Youn
Park Sang-Joon
Kim Kil-Soo
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Abstract
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The kainic acid-induced seizure mouse model is widely used in epilepsy research. In this study, we applied kainic acid to the subcutaneous injections of three different sources of DBA/2 mice to compare and evaluate the seizure response. The three mouse sources consisted of DBA/2Kor1 (Korea FDA source), DBA/2A (USA source), and DBA/2 (Japan source), and were purchased from different vendors. To compare the responses of DBA/2 mice to kainic acid injections, we examined the survival rate, seizure phenotype scoring, and behavioral changes. We also evaluated brain lesions using histopathological analysis. Following the administration of kainic acid, almost half of the cohort survived, and the seizure phenotype displayed a moderate level of sensitivity (2?~?4 out of 6). In the histopathologic analysis, there was no change in morphological features, and levels of glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba-1) increased in the kainic acid-treated groups. However, there was no difference in the neuronal nuclei (NeuN) expression level. All the data showed that the responses in the kainic acid-treated group were similar across the three strains. In conclusion, our results suggest that the three sources of DBA/2 mice (DBA/2Kor1, DBA/2A, and DBA/2B) have similar pathological responses to kainic acid-induced seizures.
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KEYWORD
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DBA/2Kor1, Kainic acid, Seizure, Brain, DBA/2 mouse
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